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TAL019
(Plasmid #32261)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pUC57-ΔBsaI
  • Backbone size w/o insert (bp) 2708
  • Modifications to backbone
    Silent valine mutation (C->G) at position 1794 to destroy alternate BsaI restriction site in pUC57

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    TAL019
  • Alt name
    K (Type III - G/NN)
  • Alt name
    γ / NNN / G
  • Insert Size (bp)
    119

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer OK163
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL019 was a gift from Keith Joung (Addgene plasmid # 32261)
  • For your References section:

    Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. 10.1038/nbt.1934 PubMed 21822241