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Plasmid 32278: TAL040
  • TAL040

  • Type I C-terminal- (T/NG)

  • ε' / SNG / T

  • 119

  • pUC57-ΔBsaI
    (Search Vector Database)

  • 2708

  • Silent valine mutation (C->G) at position 1794 to destroy alternate BsaI restriction site in pUC57

  • BbsI

  • Yes

  • BamHI

  • No

  • OK163 (5'-CGCCAGGGTTTTCCCAGTCACGAC-3') List of Sequencing Primers

  • Ampicillin

  • XL1 Blue

  • 37

  • High Copy

  • View sequences (2)
  • Keith Joung

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32278" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with