Addgene - TALEN1257 Plasmid Data

Plasmid 32280: TALEN1257

Gene/insert name:
TAL array targeting hey2
Alt name:
hey2_R (TAL 1257)
Alt name:
hey2
Species:
D. rerio (zebrafish)
Entrez Gene:
hey2 (grl, zgc:136746)
Fusion protein or tag:
3X FLAG
Terminal:
N terminal on backbone
Fusion protein or tag:
WT FOKI
Terminal:
C terminal on backbone
Vector backbone:
JDS78
(Search Vector Database)
Vector type:
Mammalian Expression ; T7
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-f List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32280" in your Materials and Methods section.