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Plasmid 32287: JDS71
  • None

  • JDS71
    (Search Vector Database)

  • Mammalian Expression ; T7

  • 6447

  • CMV

  • 3X Flag

  • N terminal on backbone

  • FOKI WT

  • C terminal on backbone

  • Ampicillin

  • XL1 Blue

  • 37

  • High Copy

  • View sequences (3)
  • Keith Joung

  • UBMTA

Comments: 

Backbone contains SHD/C 0.5 Domain
TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32287" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only