Addgene - JDS71 Plasmid Data

Plasmid 32287: JDS71

Gene/insert name:
None
Vector backbone:
JDS71
(Search Vector Database)
Vector type:
Mammalian Expression ; T7
Backbone size (bp):
6447
Promoter:
CMV
Fusion protein or tag:
3X Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
FOKI WT
Terminal:
C terminal on backbone
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Backbone contains SHD/C 0.5 Domain
TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
FOKI: WT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32287" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

32285: JDS70

32288: JDS74

32290: JDS78

1000000016: Golden Gate TALEN and TAL effector kit

14738: pBABE HAII