|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32287||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6447
Vector typeMammalian Expression ; T7
- Promoter CMV
/ Fusion Proteins
- 3X Flag (N terminal on backbone)
- FOKI WT (C terminal on backbone)
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Backbone contains SHD/C 0.5 Domain
TAL N-terminal: ∆152 (Miller et al. 2011)
TAL C-terminus: +63 (Miller et al. 2011)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:JDS71 was a gift from Keith Joung (Addgene plasmid # 32287)
For your References section:Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR. Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. 10.1038/nbt.1934 PubMed 21822241
Generated by Addgene from full sequence supplied by depositor.