|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32403||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMark Bushell
- Backbone size (bp) 6000
Vector typeMammalian Expression
- Promoter CMV promoter
/ Fusion Proteins
- ratCd4d3+4 (C terminal on insert)
- pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on insert)
- beta-lactamase (C terminal on insert)
Growth in Bacteria
Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD4d3+4-blac was a gift from Gavin Wright (Addgene plasmid # 32403 ; http://n2t.net/addgene:32403 ; RRID:Addgene_32403)
For your References section:Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487