Plasmid 32403: CD4d3+4-blac

• Gene/insert name: None
• Vector backbone: pEMCS
  (Search Vector Database)
• Backbone manufacturer: Mark Bushell
• Vector type: Mammalian Expression
• Backbone size (bp): 6000
• Promoter: CMV promoter
• Fusion protein or tag: ratCd4d3+4
• Terminal: C terminal on insert
• Fusion protein or tag: pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP)
• Terminal: C terminal on insert
• Fusion protein or tag: beta-lactamase
• Terminal: C terminal on insert
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: Unknown
• Person or lab that originally
  cloned the gene/insert: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
• Sequence: View sequences (3)
• Map: View map
• Principal Investigator: Gavin Wright
• Terms and Licenses: MTA

Comments: 

Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression.

Article: Large-scale screening for novel low-affinity extracellular protein interactions. Bushell et al (Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. PubMed)