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CMV-FFluc-GPD1L-3'UTR Full mut
(Plasmid #32489)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 32489 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPD1L 3'UTR
  • Alt name
    GPD1L
  • Species
    M. musculus (mouse)
  • Mutation
    Mutated in the entire miR-210 binding region
  • Entrez Gene
    Gpd1l (a.k.a. 2210409H23Rik, D9Ertd660e)
  • Promoter CMV
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Full mutant sequence- CAGTGTGAATGCGTGTC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-FFluc-GPD1L-3'UTR Full mut was a gift from Pere Puigserver (Addgene plasmid # 32489 ; http://n2t.net/addgene:32489 ; RRID:Addgene_32489)
  • For your References section:

    A hypoxia-induced positive feedback loop promotes hypoxia-inducible factor 1alpha stability through miR-210 suppression of glycerol-3-phosphate dehydrogenase 1-like. Kelly TJ, Souza AL, Clish CB, Puigserver P. Mol Cell Biol. 2011 Jul;31(13):2696-706. Epub 2011 May 9. 10.1128/MCB.01242-10 PubMed 21555452