Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET28-mE1
(Plasmid #32534)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32534 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E1, ubiquitin-activating enzyme
  • Alt name
    UBA1
  • Alt name
    UBE1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3177
  • Entrez Gene
    Uba1 (a.k.a. RP23-394F6.2, A1S9, AA989744, Sbx, Ube-1, Ube1x)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original mouse ubiquitin-activating enzyme cDNA was cloned by Dr. Fumiaki Yamao, National Institute of Genetics, Japan.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-mE1 was a gift from Jorge Eduardo Azevedo (Addgene plasmid # 32534 ; http://n2t.net/addgene:32534 ; RRID:Addgene_32534)
  • For your References section:

    High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1. Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sa-Miranda C, Azevedo JE. Mol Biotechnol. 2011 Oct 20. 10.1007/s12033-011-9463-x PubMed 22012022