Ttr:mRFP1
(Plasmid
#32607)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTTR1ExV3
- Total vector size (bp) 1210
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTTR:mRFP
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Ttr::RFP constructs were generated by inserting a 1-kb mRFP1 (Campbell et al., 2002)
fragment into either the NruI site of the first exon or StuI site of the second exon of
pTTR1ExV3 (Costa et al., 1990).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ttr:mRFP1 was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 32607 ; http://n2t.net/addgene:32607 ; RRID:Addgene_32607) -
For your References section:
Transthyretin mouse transgenes direct RFP expression or Cre-mediated recombination throughout the visceral endoderm. Kwon GS, Hadjantonakis AK. Genesis. 2009 Jul;47(7):447-55. 10.1002/dvg.20522 PubMed 19415627