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pSADdeltaG-GFP-ER(T2)CreER(T2)
(Plasmid #32649)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pSADdeltaG
  • Backbone manufacturer
    Addgene plasmid 32634
  • Backbone size w/o insert (bp) 15428
  • Vector type
    Mammalian Expression ; Rabies virus
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Insert Size (bp)
    700
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ER(T2)CreER(T2)
  • Insert Size (bp)
    2900

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADdeltaG-GFP-ER(T2)CreER(T2) was a gift from Edward Callaway (Addgene plasmid # 32649)
  • For your References section:

    New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM. Neuron. 2011 Aug 25;71(4):617-31. 10.1016/j.neuron.2011.07.005 PubMed 21867879