Addgene - pcDNA3β-FLAG-CBP-LD-HA Plasmid Data

Plasmid 32906: pcDNA3β-FLAG-CBP-LD-HA

Gene/insert name:
C/EBP
Alt name:
creb binding protein
Species:
M. musculus (mouse)
Entrez Gene:
Crebbp (AW558298, CBP, CBP/p300, KAT3A, p300/CBP)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
L1435A/D1436A
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Promoter:
CMV
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-Fwd List of Sequencing Primers
3' sequencing primer:
BGH-Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Tso-Pang Yao
Terms and Licenses:
MTA

Comments:

This is the catalytic deficient mutant.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. Zhao et al (Mol Cell Biol. 2005 Oct;25(19):8456-64. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32906" in your Materials and Methods section.