Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32989||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerNordeen et al., 1988
- Backbone size w/o insert (bp) 6000
Growth in Bacteria
Gene/Insert nameSiamois promoter
SpeciesX. laevis (frog)
Insert Size (bp)833
Entrez Genesia1.L (a.k.a. XELAEV_18019203mg, Xsia, sia, sia1, siamois, siamois-A)
- Promoter Siamois
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-833pSia-Luc was a gift from Sergei Sokol (Addgene plasmid # 32989 ; http://n2t.net/addgene:32989 ; RRID:Addgene_32989)
For your References section:Wnt signaling and transcriptional control of Siamois in Xenopus embryos. Fan MJ, Gruning W, Walz G, Sokol SY. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5626-31. 10.1073/pnas.95.10.5626 PubMed 9576934
Map uploaded by the depositor.