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-833pSia-Luc
(Plasmid #32989)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32989 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXP2
  • Backbone manufacturer
    Nordeen et al., 1988
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Siamois promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    833
  • Entrez Gene
    sia1.L (a.k.a. XELAEV_18019203mg, Xsia, sia, sia1, siamois, siamois-A)
  • Promoter Siamois
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer Luc_N_Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -833pSia-Luc was a gift from Sergei Sokol (Addgene plasmid # 32989 ; http://n2t.net/addgene:32989 ; RRID:Addgene_32989)
  • For your References section:

    Wnt signaling and transcriptional control of Siamois in Xenopus embryos. Fan MJ, Gruning W, Walz G, Sokol SY. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5626-31. 10.1073/pnas.95.10.5626 PubMed 9576934