Gene/insert name: Xfz8
Insert size: 1979
Species:
X. laevis (frog)
Entrez Gene:
fzd8-a
(Xfz8, frizzled8, fzd8)
Vector backbone:
pXT7Search Vector Database )
Backbone manufacturer:
R. Freeman/ S. Sokol
Vector type:
Xenopus RNA expression
Backbone size w/o insert (bp):
3050
Promoter: T7
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
Yes
5' sequencing primer:
T7
List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence:
View sequences (1)
Map:
View map
Principal Investigator:
Sergei Sokol
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
32991" in your Materials and Methods section.
To make mRNA, digest with Pst1 and transcribe with T7 RNA polymerase.