Backbone manufacturerR. Freeman/ S. Sokol
- Backbone size w/o insert (bp) 3050
Vector typeXenopus RNA expression
Growth in Bacteria
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesX. laevis (frog)
Insert Size (bp)1979
Entrez Genefzd8-a (a.k.a. Xfz8, frizzled8, fzd8)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
To make mRNA, digest with Pst1 and transcribe with T7 RNA polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Xfz8-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 32991)
For your References section:A role for Xenopus Frizzled 8 in dorsal development. Itoh K, Jacob J, Y Sokol S. Mech Dev. 1998 Jun;74(1-2):145-57. 10.1016/S0925-4773(98)00076-8 PubMed 9651509
Map uploaded by the depositor.