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pACAGW-H2B-PAGFP-AAV
(Plasmid #33000)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33000 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 4771
  • Vector type
    Mammalian Expression, AAV ; adeno associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    Grow on Stbl2 cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-PAGFP
  • Alt name
    histone2b-photoactivatable gfp
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    1116
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Tag / Fusion Protein
    • PAGFP (C terminal on insert)

Resource Information

Depositor Comments

Plasmid containing the H2B-PAGFP fusion gene was a gift from André Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071).

Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34.

There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACAGW-H2B-PAGFP-AAV was a gift from Massimo Scanziani (Addgene plasmid # 33000 ; http://n2t.net/addgene:33000 ; RRID:Addgene_33000)
  • For your References section:

    In vivo Labeling of Constellations of Functionally Identified Neurons for Targeted in vitro Recordings. Lien AD, Scanziani M. Front Neural Circuits. 2011;5:16. Epub 2011 Nov 29. 10.3389/fncir.2011.00016 PubMed 22144948