1
Plasmid 33143: pVS1
  • LacO (256 copies)

  • 10000

  • E. coli

  • Ylplac128
    (Search Vector Database)

  • Yeast Expression

  • CEN6ARSH4 element from pRS415 (Christianson et al., 1992) was amplified using primers flanked with AatII sites, digested with AatII and cloned into the AatII site of parent vector carrying 256 copies of LacO (Straight et al., 1998).

  • Ampicillin

  • Stbl2

  • 30

  • Unknown

  • LEU2

  • View sequences (1)
  • Michael Rosbash

  • UBMTA

Comments: 

Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.

For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).

pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).

pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala et al (Mol Cell. 2008 Jul 11;31(1):104-13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33143" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only