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pVS1
(Plasmid #33143)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    Ylplac128
  • Modifications to backbone
    CEN6ARSH4 element from pRS415 (Christianson et al., 1992) was amplified using primers flanked with AatII sites, digested with AatII and cloned into the AatII site of parent vector carrying 256 copies of LacO (Straight et al., 1998).
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    LacO (256 copies)
  • Species
    E. coli
  • Insert Size (bp)
    10000

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene cannot verify or guarantee the presence of all 256 copies of LacO. This element is unstable.

For use in two-plasmid-based gene-nuclear periphery tethering assay with either plasmids pAK67 (Addgene #33142).

pVS2 (Addgene #33144) contains 256 LacO repeats and a GAL-GFP-GALpA-reporter gene (pVS1 is identical but does not contain the reporter gene). The reporter was previously characterized and contains a GFP open reading frame flanked by the GAL1 promoter and GAL1 3′ UTR ([Abruzzi et al., 2006] and [Dower et al., 2004]).

pKA67 expresses both LacI-GFP and Nup49-GFP to mark the first plasmid as well as the nuclear periphery with GFP.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVS1 was a gift from Michael Rosbash (Addgene plasmid # 33143)
  • For your References section:

    The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala S, Abruzzi KC, Rosbash M. Mol Cell. 2008 Jul 11;31(1):104-13. 10.1016/j.molcel.2008.05.015 PubMed 18614049