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Plasmid 33181: pDMG8c
  • C27H6.9 3'UTR

  • C. elegans (nematode)

  • NM_001129395.1

  • C27H6.9 (C27H6.9)

  • Luciferase

  • N terminal on backbone

  • LTA sites: atacGaTa, atacGaTa

  • pIS1
    (Search Vector Database)

  • Addgene (#12179)

  • Mammalian Expression, Luciferase

  • 4085

  • XbaI

  • No

  • EagI

  • No

  • Rluc-F List of Sequencing Primers

  • EBV Reverse

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • David Bartel

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia et al (Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33181" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with