pCS247
(Plasmid #33201)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pIS2
  • Backbone manufacturer
    Addgene plasmid #12177
  • Backbone size w/o insert (bp) 3745
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    LRIG1
  • Species
    H. sapiens (human)
  • Mutation
    miR-CGCG sites: aCgCgaa, aaCgCgaa
  • GenBank ID
    NM_015541.2
  • Entrez Gene
    LRIG1 (a.k.a. DKFZp586O1624, LIG-1, LIG1)
  • Promoter SV40 early enhancer/promoter
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer Rluc-F
  • 3′ sequencing primer EBV rev primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS247 was a gift from David Bartel (Addgene plasmid # 33201)
  • For your References section:

    Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP. Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. 10.1038/nsmb.2115 PubMed 21909094