Addgene - pCS253 Plasmid Data

Plasmid 33209: pCS253

Gene/insert name:
RAP1A
Species:
H. sapiens (human)
GenBank ID:
NM_001010935.1
Entrez Gene:
RAP1A (KREV-1, KREV1, RAP1, SMGP21)
Fusion protein or tag:
Luciferase
Terminal:
N terminal on backbone
Mutation:
miR-CGCG sites: aCgCgaa, aCgCgaa
Vector backbone:
pIS2
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #12177
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
3745
Promoter:
SV40 early enhancer/promoter
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
Rluc-F List of Sequencing Primers
3' sequencing primer:
EBV Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia et al (Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33209" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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