Plasmid 33211: pCS260
  • SYNM

  • H. sapiens (human)

  • NM_145728.2


  • Luciferase

  • N terminal on backbone

  • miR-CGCG sites: aCgCgaa, aaCgCgaa

  • pIS2
    (Search Vector Database)

  • Addgene plasmid #12177

  • Mammalian Expression, Luciferase

  • 3745

  • SV40 early enhancer/promoter

  • SacI

  • No

  • SpeI

  • No

  • Rluc-F List of Sequencing Primers

  • EBV Reverse

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • David Bartel


Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia et al (Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33211" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only
This is commonly requested with