Addgene - pDMG1n Plasmid Data

Plasmid 33224: pDMG1n

Gene/insert name:
cog-1 3'UTR
Species:
C. elegans (nematode)
GenBank ID:
NM_001027093
Entrez Gene:
cog-1 (R03C1.3)
Fusion protein or tag:
Luciferase
Terminal:
N terminal on backbone
Mutation:
2nd site GU6,8
Vector backbone:
pIS1
(Search Vector Database)
Backbone manufacturer:
Addgene (#12179)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4085
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
EagI
Site destroyed during cloning:
No
5' sequencing primer:
none List of Sequencing Primers
3' sequencing primer:
EBV Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs. Garcia et al (Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 10.1038/nsmb.2115. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33224" in your Materials and Methods section.

Price: $65.00

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