Addgene - pT7-M3T1L Plasmid Data

Plasmid 33291: pT7-M3T1L

Gene/insert name:
M3
Species:
Orthoreovirus T1L
GenBank ID:
Entrez Gene:
MoV1sM3gp1 (MoV1sM3gp1)
Vector backbone:
p3E5EGFP
(Search Vector Database)
Backbone manufacturer:
made by Yoshihiro Kawaoka
Vector type:
Mammalian Expression
Promoter:
T7
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 forward List of Sequencing Primers
3' sequencing primer:
T7 terminal
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Terence Dermody
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Kobayashi et al (Cell Host Microbe. 2007 Apr 19;1(2):147-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33291" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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