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pLEW100cre-EP1-6G
(Plasmid #33344)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33344 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM
  • Backbone size w/o insert (bp) 4922
  • Vector type
    Trypanosoma brucei expression vector
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cre recombinase
  • Insert Size (bp)
    1032
  • GenBank ID
    X03453

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    John Donelson, Dept. Biochemistry, University of Iowa
  • Terms and Licenses

Depositor Comments

This plasmid is also known as pLEW100creTS-SAS.

Cre recombinase is flanked by UTR sequences to allow regulated T7 promoter-driven low level expression after cleavage by NotI and integration into a rRNA locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEW100cre-EP1-6G was a gift from George Cross (Addgene plasmid # 33344 ; http://n2t.net/addgene:33344 ; RRID:Addgene_33344)
  • For your References section:

    CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei. Scahill MD, Pastar I, Cross GA. Mol Biochem Parasitol. 2008 Jan;157(1):73-82. Epub 2007 Oct 6. 10.1016/j.molbiopara.2007.10.003 PubMed 18006158