Addgene - L57R Plasmid Data

Plasmid 34557: L57R

Gene/insert name:
L57
Alt name:
LOV2-Caspase-7 fusion
Alt name:
Caspase-7
Alt name:
Photoactivated Caspase-7
Insert size:
1200
Species:
H. sapiens (human)
Species:
A.Sativa (LOV2)
Entrez Gene:
CASP7 (RP11-211N11.6, CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
Fusion protein or tag:
mRFP
Terminal:
C terminal on insert
Mutation:
catalytic domain of caspase-7 was truncated at amino acid 57
Vector backbone:
pTriEx-3
(Search Vector Database)
Backbone manufacturer:
PMID: 12864922
Vector type:
Mammalian Expression, Bacterial Expression
Backbone size w/o insert (bp):
5000
Promoter:
CMV, T7
Cloning site 5':
NcoI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV-F, T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Kevin Truong
Terms and Licenses:
MTA
Clontech Limited Use Label License

Comments:

tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and a mRFP variant that contains T140A and M141S mutations (introduced to facilitate cloning).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34557" in your Materials and Methods section.

Price: $65.00

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