Addgene - pWarf(+) Plasmid Data

Plasmid 34562: pWarf(+)

Gene/insert name:
HRV 3C-GPA-GFP-8HIS
Vector backbone:
pET28a(+)
(Search Vector Database)
Backbone manufacturer:
EMD
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5329
Bacterial resistance(s)
Kanamycin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Jeff Abramson
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Hsieh et al (Protein Sci. 2010 Apr;19(4):868-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34562" in your Materials and Methods section.