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pMIR-Report-Luc-KLF4-K5S-Mt206A
(Plasmid #34599)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34599 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIR-Report-LUC
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLF4
  • Alt name
    GKLF
  • Alt name
    EZF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    306
  • Mutation
    The KLF4 K5S region was mutated at the miR-206 binding site: the seed binding region was mutated from CATTCC to ACTCTC.
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI (destroyed during cloning)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer use KLF4 or firefly luciferase internal primers
  • 3′ sequencing primer use KLF4 or firefly luciferase internal primers
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is a translation reporter. PCR primers were used to introduce mutations at the microRNA seed binding region of the K5S translational control element. The final constructs was confirmed by sequence analysis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-Luc-KLF4-K5S-Mt206A was a gift from Michael Ruppert (Addgene plasmid # 34599)
  • For your References section:

    A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959