Addgene - pMIR-Report-Luc-KLF4-K5S-Mt206B Plasmid Data

Plasmid 34600: pMIR-Report-Luc-KLF4-K5S-Mt206B

Gene/insert name:
KLF4
Alt name:
GKLF
Alt name:
EZF
Insert size:
306
Species:
H. sapiens (human)
GenBank ID:
AF105036
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Mutation:
The KLF4 K5S region was mutated at the miR-206 binding site: the seed binding region was mutated from CATTCC to CTATCC.
Vector backbone:
pMIR-Report-LUC
(Search Vector Database)
Backbone manufacturer:
Ambion
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
6470
Promoter:
CMV
Cloning site 5':
PmeI
Site destroyed during cloning:
Yes
Cloning site 3':
PmeI
Site destroyed during cloning:
Yes
5' sequencing primer:
use KLF4 or firefly luciferase internal primers List of Sequencing Primers
3' sequencing primer:
use KLF4 or firefly luciferase internal primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Ruppert
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

This is a translation reporter. PCR primers were used to introduce mutations at the microRNA seed binding region of the K5S translational control element. The final constructs was confirmed by sequence analysis.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin et al (Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34600" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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