Addgene - p413TEF-PKM2 Plasmid Data

Plasmid 34608: p413TEF-PKM2

Gene/insert name:
PKM2
Insert size:
1586
Species:
H. sapiens (human)
GenBank ID:
NM002654.4
Entrez Gene:
PKM (CTHBP, OIP3, PK3, PKM2, TCB, THBP1)
Vector backbone:
P413TEF
(Search Vector Database)
Backbone manufacturer:
ATCC#87362 (PMID:7737504)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5557
Promoter:
TEF
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBluescriptSK List of Sequencing Primers
3' sequencing primer:
CYC1
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
HIS3
Sequence: View sequences (2)
Principal Investigator:
Markus Ralser
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Bluemlein et al (Oncotarget. 2011 May;2(5):393-400. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34608" in your Materials and Methods section.