PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34615||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namesynthetic TEAD luciferase reporter
Alt nameYAP/TAZ luciferase reporter
Alt namecontains the minimal chicken TnT promoter
Insert Size (bp)360
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer luciferase-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene sequencing detected a 7 nucleotide insertion at position 107 relative to the author's insert sequence. This results in the loss of a MscI restriction site but does not alter the function of the plasmid.
Please click http://www.addgene.org/34615/notes/ for general advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xGTIIC lux.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34615 ; http://n2t.net/addgene:34615 ; RRID:Addgene_34615)
For your References section:Role of YAP/TAZ in mechanotransduction. Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, Piccolo S. Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137. 10.1038/nature10137 PubMed 21654799