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Ai27
(Plasmid #34630)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34630 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Rosa26 Targeting Vector
  • Backbone size w/o insert (bp) 15658
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAG-Floxed hChR2(H134R)-tdTomato
  • Insert Size (bp)
    2367
  • Mutation
    The His at 134 has been mutated to R
  • Promoter CAG
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer pCAG-F, dsRed1-C
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ai27 was a gift from Hongkui Zeng (Addgene plasmid # 34630)
  • For your References section:

    A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Madisen L, Mao T, Koch H, Zhuo JM, Berenyi A, Fujisawa S, Hsu YW, Garcia AJ 3rd, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, Boyden ES, Buzsaki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner EE, Zeng H. Nat Neurosci. 2012 Mar 25;15(5):793-802. doi: 10.1038/nn.3078. 10.1038/nn.3078 PubMed 22446880