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Plasmid 34971: pDS257
  • Mid2(SS/TM)-GFP-LOVpep

  • 2236

  • S. cerevisiae (budding yeast); A. sativa

  • YIPlac128
    (Search Vector Database)

  • Yeast Expression

  • 4263

  • PGAL-Mid2(SS/TM)-GFP-LOVpep-TCYC1 cassette inserted into YIPlac128

  • SacI

  • No

  • SphI

  • Yes

  • M13 fwd List of Sequencing Primers

  • M13 rev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • LEU2

  • GFP(S65T) CDS and other sequences from pGREG plasmids (EUROSCARF). A. sativa LOV2 CDS from Kevin Gardner, UT Southwestern Medical Center

  • View sequences (3)
  • View map

  • Genbank sequence (application/octet-stream)

  • Michael Glotzer

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland et al (Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34971" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only