Addgene - pDS257 Plasmid Data

Plasmid 34971: pDS257

Gene/insert name:
Mid2(SS/TM)-GFP-LOVpep
Insert size:
2236
Species:
S. cerevisiae (budding yeast)
Species:
A. sativa
Vector backbone:
YIPlac128
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
4263
Modifications to Backbone:
PGAL-Mid2(SS/TM)-GFP-LOVpep-TCYC1 cassette inserted into YIPlac128
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
SphI
Site destroyed during cloning:
Yes
5' sequencing primer:
M13 fwd List of Sequencing Primers
3' sequencing primer:
M13 rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
LEU2
Person or lab that originally
cloned the gene/insert:
GFP(S65T) CDS and other sequences from pGREG plasmids (EUROSCARF). A. sativa LOV2 CDS from Kevin Gardner, UT Southwestern Medical Center
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Genbank sequence (application/octet-stream)
Principal Investigator:
Michael Glotzer
Terms and Licenses:
MTA

Comments:

The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland et al (Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34971" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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