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pDS257
(Plasmid #34971)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    YIPlac128
  • Backbone size w/o insert (bp) 4263
  • Modifications to backbone
    PGAL-Mid2(SS/TM)-GFP-LOVpep-TCYC1 cassette inserted into YIPlac128
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Mid2(SS/TM)-GFP-LOVpep
  • Species
    S. cerevisiae (budding yeast); A. sativa
  • Insert Size (bp)
    2236

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SphI (destroyed during cloning)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS257 was a gift from Michael Glotzer (Addgene plasmid # 34971)
  • For your References section:

    TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287