|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34985||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backbonePGAL–ePDZb1–TCYC1 cassette inserted into YIPlac211
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
- Promoter GAL1
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (destroyed during cloning)
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDS296 was a gift from Michael Glotzer (Addgene plasmid # 34985 ; http://n2t.net/addgene:34985 ; RRID:Addgene_34985)
For your References section:TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287