|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35188||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 7009
Modifications to backboneAddition of TALE Transcription Factor motif with +63 C Terminus and VP64 domain. Also includes a T2A skip peptide followed by GFP under control of the same CMV promoter.
Vector typeMammalian Expression, TALEN
Selectable markersNeomycin (select with G418) ; XGAL/IPTG
Growth in Bacteria
Growth instructionsLB + Antibiotics
Copy numberHigh Copy
Gene/Insert nameTAL Effector-VP64-T2A-eGFP
Insert Size (bp)1581
Mutation+63 C Terminus, VP64 Domain, T2A-GFP
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (not destroyed)
- 3′ cloning site BsmBI (not destroyed)
- 5′ sequencing primer GTAACAGCGGTAGAGGCAGTG
- 3′ sequencing primer CGTCCACCAAGACATGCCAAC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe modified the MR15 backbone provided by the Porteus lab at the Stanford University School of Medicine.
Terms and Licenses
MR15 is a designation vector for the Cermak et al Golden Gate system designed for expression in mammalian cells.
Please acknowledge the principal investigator, Dr. Gang Bao, and include this article in your citations if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35188" in your Materials and Methods section.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTALE64G was a gift from Gang Bao (Addgene plasmid # 35188 ; http://n2t.net/addgene:35188 ; RRID:Addgene_35188)