- Backbone size w/o insert (bp) 4374
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
Alt nameJBEI Part ID: JPUB_000117
Insert Size (bp)717
- Promoter ProE
- Cloning method Restriction Enzyme
- 5′ sequencing primer GFP-R
- 3′ sequencing primer GFP-F (Common Sequencing Primers)
Terms and Licenses
JBEI Part ID: JPUB_000117; Origin: BBR1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBbB4k-GFP was a gift from Jay Keasling (Addgene plasmid # 35351)
For your References section:BglBrick vectors and datasheets: A synthetic biology platform for gene expression. Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. J Biol Eng. 2011 Sep 20;5:12. 10.1186/1754-1611-5-12 PubMed 21933410
Map generated by Addgene from full sequence supplied by depositor.