Addgene - TALE6 Plasmid Data

Plasmid 35421: TALE6

Gene/insert name:
TALE6
Alt name:
dTALE6
Insert size:
3531
Species:
Synthetic
Fusion protein or tag:
EGFP
Terminal:
C terminal on insert
Vector backbone:
pLenti
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
9204
Promoter:
EF-1a
Cloning site 5':
unknown
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EF1a-fwd, zTALE-F List of Sequencing Primers
3' sequencing primer:
EGFP-N, zTALE-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Feng Zhang
Terms and Licenses:
MTA

Comments:

Note that though this plasmid contains the sh_ble gene, the Zhang lab has not tested whether this plasmid confers Zeocin selection.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al (Nat Biotechnol. 2011 Jan 19. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35421" in your Materials and Methods section.