Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pRSII414
(Plasmid #35452)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35452 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS41x
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 4787
  • Vector type
    Yeast centromere vector
  • Promoter None
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Forward, T7
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel J. Lew, Duke University Medical Center Chandra Tucker, Duke University (now at University of Colorado, Boulder)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSII414 was a gift from Steven Haase (Addgene plasmid # 35452 ; http://n2t.net/addgene:35452 ; RRID:Addgene_35452)
  • For your References section:

    New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222