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pcDNA3/Myc-DNMT3L
(Plasmid #35523)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35523 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DNMT3L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1161
  • GenBank ID
    NM_175867.2
  • Entrez Gene
    DNMT3L
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see comments (not destroyed)
  • 3′ cloning site see comments (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.

The full-length cDNA for human DNMT3L was cloned into EcoRI and XhoI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/Myc-DNMT3L was a gift from Arthur Riggs (Addgene plasmid # 35523 ; http://n2t.net/addgene:35523 ; RRID:Addgene_35523)
  • For your References section:

    Physical and functional interactions between the human DNMT3L protein and members of the de novo methyltransferase family. Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F. J Cell Biochem. 2005 Aug 1;95(5):902-17. 10.1002/jcb.20447 PubMed 15861382