Addgene - MSCV.IRES.hCD4 Plasmid Data

Plasmid 35712: MSCV.IRES.hCD4

Gene/Insert 1
Gene/insert name:
IRES
Insert size:
595
Gene/Insert 2
Gene/insert name:
hCD4
Insert size:
1381
Species:
H. sapiens (human)
Vector backbone:
MSCV 2.2
(Search Vector Database)
Vector type:
Retroviral
Backbone size w/o insert (bp):
6505
Cloning information for Gene/Insert 1
Cloning site 5':
EcorI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcorI
Site destroyed during cloning:
Unknown
5' sequencing primer:
TGG CTC TCC TCA AGC GTA TT List of Sequencing Primers
3' sequencing primer:
CCT CAC ATT GCC AAA AGA CG
Cloning information for Gene/Insert 2
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
GCG AAA ACA GGA AAG TTG CAT C List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
Thomas Graf
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Patricia Ernst
Terms and Licenses:
MTA

Comments:

Please note that the original plasmid was described in Xie et al., 2004 Cell (PubMed ID - 15163413).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35712" in your Materials and Methods section.