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pSPTg.T2FpAXK (#52)
(Plasmid #35962)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35962 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Tie2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000

Gene/Insert 2

  • Gene/Insert name
    SV40 pA
  • Insert Size (bp)
    300

Gene/Insert 3

  • Gene/Insert name
    Tie2 enhancer
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600

Resource Information

  • Terms and Licenses

Depositor Comments

This vector contains Tie2 promoter, SV40 polyA signal and Tie2 minimum enhancer fragment. MCS (HindIII and NotI) are located between the promoter and pA signal where you can insert your cDNA as indicated. DNA fragments can be excised by SalI digest from injection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPTg.T2FpAXK (#52) was a gift from Thomas Sato (Addgene plasmid # 35962 ; http://n2t.net/addgene:35962 ; RRID:Addgene_35962)
  • For your References section:

    Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Schlaeger TM, Bartunkova S, Lawitts JA, Teichmann G, Risau W, Deutsch U, Sato TN. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63. 10.1073/pnas.94.7.3058 PubMed 9096345