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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3519
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Vector typecloning vector
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefibronectin type III domain containing 5
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Alt nameFndc5, PeP, Pxp, C87088, AI836596, 1500001L03Rik
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SpeciesM. musculus (mouse)
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Insert Size (bp)630
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GenBank IDNM_027402
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Entrez GeneFndc5 (a.k.a. 1500001L03Rik, PeP, Pxp)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Fndc5 gene has a high GC content and therefore may be difficult to re-clone by PCR, restriction digest should be used in re-cloning attempts.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR-blunt-TOPO-FNDC5 was a gift from Bruce Spiegelman (Addgene plasmid # 35970 ; http://n2t.net/addgene:35970 ; RRID:Addgene_35970) -
For your References section:
A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Bostrom EA, Choi JH, Long JZ, Kajimura S, Zingaretti MC, Vind BF, Tu H, Cinti S, Hojlund K, Gygi SP, Spiegelman BM. Nature. 2012 Jan 11;481(7382):463-8. doi: 10.1038/nature10777. 10.1038/nature10777 PubMed 22237023