Addgene - ptp4a3_L (TAL 3000) Plasmid Data

Plasmid 35992: ptp4a3_L (TAL 3000)

Gene/insert name:
TAL array targeting ptp4a3
Alt name:
ptp4a3
Species:
D. rerio (zebrafish)
Entrez Gene:
ptp4a3 (wu:fc54b05, wu:fv52d11, zgc:77109)
Fusion protein or tag:
3X Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
WT FOKI
Terminal:
C terminal on backbone
Vector backbone:
JDS 74
(Search Vector Database)
Vector type:
Mammalian Expression ; T7
Cloning site 5':
NA
Site destroyed during cloning:
Unknown
Cloning site 3':
NA
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-f List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander et al (Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35992" in your Materials and Methods section.