unc5bCD4d3+4-His
(Plasmid #36151)

Loading...
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    unc5b
  • Alt name
    unc5b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1150
  • Mutation
    L2V
  • Entrez Gene
    unc5b (a.k.a. unc5h2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ratCD4d3+4 (C terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Terms and Licenses

Depositor Comments

This plasmid contains an intentional L2V mutation in the unc5b sequence to create a consensus Kozak sequence.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    unc5bCD4d3+4-His was a gift from Gavin Wright (Addgene plasmid # 36151)
  • For your References section:

    A cell surface interaction network of neural leucine-rich repeat receptors. Sollner C, Wright GJ. Genome Biol. 2009;10(9):R99. Epub 2009 Sep 18. 10.1186/gb-2009-10-9-r99 PubMed 19765300