Addgene - pc3xTC20-alphaTubulin Plasmid Data

Plasmid 36327: pc3xTC20-alphaTubulin

Gene/insert name:
alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling
Alt name:
3xTC20
Insert size:
1484
Species:
H. sapiens (human), Synthetic
Fusion protein or tag:
3xTC20
Terminal:
N terminal on insert
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Promoter:
CMV
Cloning method:
Unknown
5' sequencing primer:
T7 Forward List of Sequencing Primers
3' sequencing primer:
BGH Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Amy Palmer
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FACS-based selection of tandem tetracysteine peptides with improved ReAsH brightness in live cells. Van et al (Chembiochem. 2010 Mar 1;11(4):489-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36327" in your Materials and Methods section.