Addgene - pmEGFP-16 Plasmid Data

Plasmid 36411: pmEGFP-16

Gene/insert name:
None
Vector backbone:
pEGFP
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Modifications to Backbone:
hexa casette of enhanced GFP (mammalian codon optimized) with monomerizing A206K mutation
Promoter:
CMV
3' sequencing primer:
AmpR_promoter
Fusion protein or tag:
6X mEGFP
Terminal:
C terminal on backbone
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
pmEGFP-15_genbank (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Benjamin Glick
Terms and Licenses:
MTA

Comments:

Plasmid carrying a hexa casette of monomeric (A206K) EGFP for C-terminal tagging.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36411" in your Materials and Methods section.