Vector backbonepEGFP-1 (NOT pEGFP-N1)
Modifications to backbonehexa casette of enhanced GFP (mammalian codon optimized) with monomerizing A206K mutation
Vector typeFluorescent protein cassette for sub-cloning
- Promoter No promoter
/ Fusion Protein
- 6X mEGFP (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 3′ sequencing primer AmpR_promoter (Common Sequencing Primers)
Terms and Licenses
Plasmid carrying a hexa casette of monomeric (A206K) EGFP for C-terminal tagging.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmEGFP-16 was a gift from Benjamin Glick (Addgene plasmid # 36411)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.