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mTert-pBabe-puro
(Plasmid #36413)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36413 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone manufacturer
    Bob Weinberg (Addgene Plasmid #1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTERT
  • Species
    M. musculus (mouse)
  • GenBank ID
    BC127068.1
  • Entrez Gene
    Tert (a.k.a. EST2, TCS1, TP2, TR, TRT)
  • Promoter LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer mTERT 1: 5′-TTCGAACAGCAAACCAACAG-3′
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTert-pBabe-puro was a gift from Marta Alvarez & Joseph Bidwell (Addgene plasmid # 36413 ; http://n2t.net/addgene:36413 ; RRID:Addgene_36413)
  • For your References section:

    Immortalization and characterization of osteoblast cell lines generated from wild-type and Nmp4-null mouse bone marrow stromal cells using murine telomerase reverse transcriptase (mTERT). Alvarez MB, Childress P, Philip BK, Gerard-O'Riley R, Hanlon M, Herbert BS, Robling AG, Pavalko FM, Bidwell JP. J Cell Physiol. 2012 May;227(5):1873-82. doi: 10.1002/jcp.22915. 10.1002/jcp.22915 PubMed 21732358