Addgene - EGFP-Abr R683A N795A Plasmid Data

Plasmid 36417: EGFP-Abr R683A N795A

Gene/insert name:
active BCR-related
Alt name:
ABR isoform A
Species:
H. sapiens (human)
Entrez Gene:
ABR (MDB)
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Mutation:
R683A N795A
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning method:
Unknown
5' sequencing primer:
EGFP-C List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Mutagenesis was done on EGFP-Abr R683A obtained from EunJoon Kim, KAIST, Korea
Sequence: View sequences (2)
Principal Investigator:
John Groffen
Principal Investigator:
Nora Heisterkamp
Terms and Licenses:
MTA

Comments:

This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho et al (Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36417" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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