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pGEX6P1_EHD1_438-534
(Plasmid #36459)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36459 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 5284
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EHD1 EH domain
  • Alt name
    EH domain containing 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    deleted amino acids 1-437 from full-length construct
  • GenBank ID
    NM_006795
  • Entrez Gene
    EHD1 (a.k.a. H-PAST, HPAST1, PAST, PAST1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original clone which was used as a template was purchased from the company BioCat, Heidelberg, Germany.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1_EHD1_438-534 was a gift from Yvonne Groemping (Addgene plasmid # 36459 ; http://n2t.net/addgene:36459 ; RRID:Addgene_36459)