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Browse > Keith Joung > Reyon et al > TAL2383
Plasmid 36792: TAL2383

  • AKT2-TALEN_Right

  • AKT2

  • 1640

  • H. sapiens (human)

  • Akt2 (2410016A19Rik, AW554154, PKB, PKBbeta)

  • 3X Flag

  • N terminal on backbone

  • WT FOKI

  • C terminal on backbone

  • JDS74
    (Search Vector Database)

  • Joung lab (Addgene Plasmid # 32288)

  • Mammalian, T7 for in vitro mRNA synthesis

  • 6447

  • CMV

  • BsmBI

  • Yes

  • BsmBI

  • Yes

  • CMV-F List of Sequencing Primers

  • BGH-rev

  • Ampicillin

  • XL1 Blue

  • 37

  • High Copy

  • View sequences (2)

  • Keith Joung

  • MTA

Comments: 

Target binding site: TGCCAGCCATCCTGTGAG

It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FLASH assembly of TALENs for high-throughput genome editing. Reyon et al (Nat Biotechnol. 2012 Apr 8. doi: 10.1038/nbt.2170. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36792" in your Materials and Methods section.

Price: $65.00

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