Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCS2-myc-gfp-dSH2
(Plasmid #36871)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36871 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 5466
  • Vector type
    Mammalian Expression, Mouse Targeting ; xenopus/mammalian/avian/zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Src homology-2 domain (dSH2)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Src (a.k.a. AW259666, pp60c-src)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The depositor notes that the discrepancies between the full sequence and the Addgene QC sequences do not affect the function of this sensor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-myc-gfp-dSH2 was a gift from Timothy Gomez (Addgene plasmid # 36871 ; http://n2t.net/addgene:36871 ; RRID:Addgene_36871)
  • For your References section:

    Src-dependent tyrosine phosphorylation at the tips of growth cone filopodia promotes extension. Robles E, Woo S, Gomez TM. J Neurosci. 2005 Aug 17;25(33):7669-81. 10.1523/JNEUROSCI.2680-05.2005 PubMed 16107653