pCS2-myc-gfp-dSH2
(Plasmid #36871)

• Depositing Lab: Timothy Gomez
• Publication: Robles et al J Neurosci. 2005 Aug 17;25(33):7669-81.

Backbone

• Vector backbone: pCS2
• Backbone manufacturer: RZPD
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 5466
• Vector type: Mammalian Expression, Mouse Targeting ; xenopus/mammalian/avian/zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Src homology-2 domain (dSH2)
• Species: M. musculus (mouse)
• Entrez Gene: Src (a.k.a. pp60c-src)
• Promoter: CMV
• Tags / Fusion Proteins:
  • myc (N terminal on insert)
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor notes that the discrepancies between the full sequence and the Addgene QC sequences do not affect the function of this sensor.