pCMMP-2xmyc-Bcl2A1-IRES-mRFP
(Plasmid #36973)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCMMP-MCS-IRES-mRFP
  • Backbone manufacturer
    Addgene plasmid #36972
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    BCL2A1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BCL2A1 (a.k.a. ACC-1, ACC-2, BCL2L5, BFL1, GRS, HBPA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xMYC (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Sugden lab plasmid p3382

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-2xmyc-Bcl2A1-IRES-mRFP was a gift from Bill Sugden (Addgene plasmid # 36973)
  • For your References section:

    The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. Pratt ZL, Zhang J, Sugden B. J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. 10.1128/JVI.06966-11 PubMed 22318153