Addgene - pSUPER.retro.puro-THAP11-C Plasmid Data

Plasmid 36994: pSUPER.retro.puro-THAP11-C

Gene/insert name:
THAP11 shRNA
shRNA sequence:
UGAUGGAAGUGAAGAUGAA
Species:
H. sapiens (human)
Entrez Gene:
THAP11 (HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
Vector backbone:
pSuper.Retro.Puro
(Search Vector Database)
Backbone manufacturer:
OligoEngine
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
6349
Cloning site 5':
BglII
Site destroyed during cloning:
Yes
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Debu Chakravarti
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker et al (Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36994" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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