Addgene - pBABE-puro-THAP11-rescue-3xFLAG Plasmid Data

Plasmid 36998: pBABE-puro-THAP11-rescue-3xFLAG

Gene/insert name:
THAP11
Insert size:
1029
Species:
H. sapiens (human)
Entrez Gene:
THAP11 (HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
Fusion protein or tag:
3xFLAG
Terminal:
C terminal on insert
Mutation:
Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC
Vector backbone:
pBABE-Puro
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid #1764
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
5' sequencing primer:
pBABE_5 List of Sequencing Primers
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE_5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (3)
Principal Investigator:
Debu Chakravarti
Terms and Licenses:
MTA

Comments:

3xFLAG-THAP11 was subcloned from Addgene plasmids #37001

Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA
and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker et al (Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36998" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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